Proceedings of the National Academy of Sciences - PNAS, 2014-07, Vol.111 (27), p.9941-9946
2014
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- Autor(en) / Beteiligte
- Titel
- Functional recovery with recombinant human IGF1 treatment in a mouse model of Rett Syndrome
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2014-07, Vol.111 (27), p.9941-9946
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2014
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Rett Syndrome is a neurodevelopmental disorder that arises from mutations in the X-linked gene methyl-CpG binding protein 2 (MeCP2). MeCP2 has a large number of targets and a wide range of functions, suggesting the hypothesis that functional signaling mechanisms upstream of synaptic and circuit maturation may contribute to our understanding of the disorder and provide insight into potential treatment. Here, we show that insulin-like growth factor-1 (IGF1) levels are reduced in young male Mecp2 -null (Mecp2 ⁻/ʸ) mice, and systemic treatment with recombinant human IGF1 (rhIGF1) improves lifespan, locomotor activity, heart rate, respiration patterns, and social and anxiety behavior. Furthermore, Mecp2 -null mice treated with rhIGF1 show increased synaptic and activated signaling pathway proteins, enhanced cortical excitatory synaptic transmission, and restored dendritic spine densities. IGF1 levels are also reduced in older, fully symptomatic heterozygous (Mecp2 ⁻/⁺) female mice, and short-term treatment with rhIGF1 in these animals improves respiratory patterns, reduces anxiety levels, and increases exploratory behavior. In addition, rhIGF1 treatment normalizes abnormally prolonged plasticity in visual cortex circuits of adult Mecp2 ⁻/⁺ female mice. Our results provide characterization of the phenotypic development of Rett Syndrome in a mouse model at the molecular, circuit, and organismal levels and demonstrate a mechanism-based therapeutic role for rhIGF1 in treating Rett Syndrome.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1311685111Titel-ID: cdi_fao_agris_US201600143670
- Format
- –
- Schlagworte
- Animals, Behavior, Animal, Behavioral neuroscience, Binding sites, Biological Sciences, Brain, Disease Models, Animal, Female, Humans, Insulin-Like Growth Factor I - pharmacology, Insulin-Like Growth Factor I - therapeutic use, Intellectual disability, Male, Medical treatment, Methyl-CpG-Binding Protein 2 - genetics, Mice, Mice, Inbred C57BL, Mice, Knockout, Mutation, Neurological disorders, Neurons, Phenotypes, Post hoc, Proteins, Recombinant Proteins - pharmacology, Recombinant Proteins - therapeutic use, Respiration, Rett Syndrome - drug therapy, Rett Syndrome - genetics, Rodents, Signal Transduction, T tests, Vehicles, Visual Cortex - drug effects, Visual Cortex - physiopathology