Details

Autor(en) / Beteiligte

• PICKENS, K. A.
• CHENG, Z. M.
• TRIGIANO, R. N.

Titel

AXILLARY BUD PROLIFERATION AND ORGANOGENESIS OF EUPHORBIA PULCHURRIMAWINTER ROSE

Ist Teil von

• In vitro cellular & developmental biology. Plant, 2005-11, Vol.41 (6), p.770-774

Ort / Verlag

Tissue Culture Association

Erscheinungsjahr

2005

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Protocols for both axillary bud proliferation and shoot organogenesis of Euphorbia pulchurrima Winter Rose™ were developed using terminal buds and leaf tissues. Greenhouse-grown terminal buds were placed on Murashige–Skoog (MS) basal medium supplemented with various concentrations of either benzlyaminopurine (BA) or thidiazuron (TDZ). Explants produced the greatest number of axillary buds on media containing between 2.2 and 8.8 μM BA. The number of explants that produced axillary buds increased with increasing BA concentration. TDZ at concentrations between 2.3 and 23.0 μM caused hyperhydricity of shoots and were not effective in promoting shoot proliferation. The most calluses and shoots were produced from leaf midvein sections from in vitro grown plants placed on the medium containing 8.8–13.3 μM BA and 17.1 μM indole-3-acetic acid (IAA) for 1 mo. before transferring to the medium containing only BA. Adventitious buds were produced only from red-pigmented callus, and explants that produced callus continued to produce adventitious shoots in the presence of IAA. Five-mo.-old shoots derived from shoot culture or organogenesis rooted readily in artificial soil with or without treatment with indolebutyric acid, and were acclimatized in the greenhouse.