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Molecular detection of an isolate of Cucumber mosaic virus infecting Oxalis corymbosa in Aligarh, India
Ist Teil von
Archiv für Phytopathologie und Pflanzenschutz, 2013-09, Vol.46 (15), p.1816-1820
Ort / Verlag
Abingdon: Taylor & Francis
Erscheinungsjahr
2013
Link zum Volltext
Quelle
Taylor & Francis Online Single Subscriptions
Beschreibungen/Notizen
Plants of Oxalis corymbosa displaying typical viral symptoms were observed during spring 2010 in plant nurseries in central India. The virus was mechanically transmissible to indicator plants and induced symptoms similar to those incited by Cucumber mosaic virus (CMV). Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 28–30 nm in diameter. Molecular detection methods allowed the identification of the pathogen as a CMV. The results were confirmed by reverse transcription polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. The coat protein gene was 95 and 99% identical at the nucleotide and amino acid levels, respectively, to the published sequences of CMV, high amino acid sequence homology in multiple alignment analysis and closest phylogenetic relationship with Lucknow isolate (JX570732) of CMV. This represents the first record for CMV-infecting O. corymbosa in Aligarh India.