Multiplex Real-Time PCR for Detection of an Epidemic KPC-Producing Klebsiella pneumoniae ST258 Clone
2012
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- Autor(en) / Beteiligte
- Titel
- Multiplex Real-Time PCR for Detection of an Epidemic KPC-Producing Klebsiella pneumoniae ST258 Clone
- Ist Teil von
- Antimicrobial Agents and Chemotherapy, 2012-06, Vol.56 (6), p.3444-3447
- Ort / Verlag
- United States: American Society for Microbiology
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0066-4804eISSN: 1098-6596DOI: 10.1128/AAC.00316-12Titel-ID: cdi_fao_agris_US201500006457
- Format
- –
- Schlagworte
- Anti-Bacterial Agents - pharmacology, antibiotic resistance, Bacterial Proteins, Bacterial Proteins - genetics, beta-Lactamases, beta-Lactamases - genetics, carbapenemase, carbapenems, Carbapenems - pharmacology, Clinical isolates, clones, Drug Resistance, Multiple, Bacterial - genetics, Epidemics, Epidemiology and Surveillance, genes, Klebsiella pneumoniae, Klebsiella pneumoniae - drug effects, Klebsiella pneumoniae - enzymology, Klebsiella pneumoniae - genetics, microbial detection, monitoring, pathogen identification, Polymerase chain reaction, quantitative polymerase chain reaction, Real-Time Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction - methods