Details

Autor(en) / Beteiligte

• Gomez de Balugera, Z
• Goicolea, M.A
• Barrio, R.J

Titel

Determination of 4-chloroaniline and 4-chlorophehyl urea in honey by liquid chromatography with electrochemical detection

Ist Teil von

• Journal of liquid chromatography & related technologies, 1997-01, Vol.20 (10), p.1591-1603

Ort / Verlag

Colchester: Taylor & Francis Group

Erscheinungsjahr

1997

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A liquid chromatographic technique with amperometric detection was developed for determining diflubenzuron metabolites, 4-chlorophenyl urea and 4-chloroaniline, using a Spherisorb C 18 column 5μm (4.1 × 250 mm). A mobile phase of acetonitrile / citrate-disodium hydrogenphosphate buffer, 30:70, at pH=7.0, was pumped at a flow rate of 1 mL min −1 . The amperometric detector, equipped with a glassy carbon working electrode was operated at 1100 mV vs Ag/AgCl. The method showed a limit of detection of 0.46 μg 1 −1 for 4-chlorophenyl urea and 0.20 μg 1 −1 for 4-chloroaniline. Reproducibility in terms of relative standard deviation ranged between 1.24 and 4.50 % for the 4-chlorophenyl urea and 1.60 and 3.93% for the 4-chloroaniline. The method was applied to the determination of both metabolites in honey. The prior extraction of the two substances from honey was established using different solid-liquid extraction phases.