Details

Autor(en) / Beteiligte

• Lloyd, R.M. Jr
• Burns, D.A
• Huong, J.T
• Mathis, R.L
• Winters, M.A
• Tanner, M
• De La Rosa, A
• Yen-Lieberman, B
• Armstrong, W
• Taege, A
• McClernon, D.R
• Wetshtein, J.L
• Friedrich, Brian M
• Ferguson, Monique R
• O'Brien, William
• Feorino, P.M
• Holodniy, M

Titel

Dried-Plasma Transport Using a Novel Matrix and Collection System for Human Immunodeficiency Virus and Hepatitis C Virus Virologic Testing

Ist Teil von

• Journal of Clinical Microbiology, 2009-05, Vol.47 (5), p.1491-1496

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• A novel method for the collection and transportation of dried-blood-plasma samples, SampleTanker (ST), was developed and compared to standard shipping protocols for frozen-plasma specimens containing human immunodeficiency virus type 1 (HIV-1) and/or hepatitis C virus (HCV). Matched frozen and dried 1-ml EDTA-containing plasma samples were collected and analyzed by several molecular-based virologic assays. After addition of 1.175 ml of reconstitution buffer, 1.035 ml of dried plasma was recovered. Mean intra-assay variances were 0.05, 0.05, and 0.06 log₁₀ copies/ml for the Versant, Amplicor, and NucliSens QT HIV-1 load assays, respectively (P, not significant). However, mean HIV-1 viral load was consistently reduced in dried samples by 0.32 to 0.51 log₁₀ copies/ml, depending on assay type (P < 0.05). Infectious HIV-1 was not recovered from dried ST plasma. There was no significant difference in HIV-1 viral load results obtained using ST after 8 weeks of storage at ambient temperature. Compared to frozen plasma, HIV-1 genotypic results were >99% concordant at the nucleotide and amino acid levels, as well as for resistance-associated mutations. We further demonstrated successful detection of multiple analytes, including HIV-1 viral load, HIV-1 antiretroviral resistance genotype, and HCV genotype, from a single ST unit. Dried plasma collected with ST yielded comparable results to frozen samples for multiple-analyte clinical testing. As such, ST could be a useful alternative for virologic tests and clinical trials worldwide by significantly diminishing transportation cost and the sample volume restrictions associated with dried-blood-spot technology.