Details

Autor(en) / Beteiligte

• Rossi-Zalaf, Luciana S
• Alves, Sérgio B
• Vieira, Solange A

Titel

Efeito de meios de cultura na virulência de Hirsutella thompsonii (Fischer) (Deuteromycetes) para o controle Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae)

Ist Teil von

• Neotropical entomology, 2008, Vol.37 (3), p.312-320

Erscheinungsjahr

2008

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The virulence of Hirsutella thompsonii (Fischer) to Brevipalpus phoenicis (Geijskes) was evaluated in laboratory, grown on complete and solid culture media (MC-S); complete and liquid culture media (MC-L); rice (APC) and powdered rice (APC-SM). Adults were confined to arenas prepared with citrus leaves in acrylic dishes containing water-agar. Conidial suspensions were prepared at different concentration (3.2 x 10⁵ to 1 x 10⁷ spores/ml) and applied on mites to establish the table curve-response on fourth day. For field evaluation, adults were maintained in arenas prepared with fruits which were placed in plants. In this test, four treatments were tried: H. thompsonii cultured on rice (APC) at two concentrations (20 kg/ha and 10 kg/ha), H. thompsonii produced by liquid fermentation (MC-L) (5 L/ha) and control (sterile water). Adult survival, number of eggs and nymphs per fruit were observed 10 and 20 days after the fungus application. The lowest LC₂₅ value calculated was from pathogen produced in MC-S (1.9 x 10⁵ conidia/ml).The LC₂₅ values calculated to APC and APC-SM did not differ statistically. The LC₂₅ values to MC-L and MC-S were 1.9 x 10⁶ infective cells/ml and 2.2 x 10⁵ conidia/ml. In the field, concentration and time to death differed between treatments and control. The applications resulted in reduction of adult survival and number of eggs.