Details

Autor(en) / Beteiligte

• Burton, Rachel A
• Wilson, Sarah M
• Hrmova, Maria
• Harvey, Andrew J
• Shirley, Neil J
• Medhurst, Anne
• Stone, Bruce A
• Newbigin, Edward J
• Bacic, Antony
• Fincher, Geoffrey B

Titel

Cellulose Synthase-Like CslF Genes Mediate the Synthesis of Cell Wall (1,3;1,4)-{szligbeta}-D-Glucans

Ist Teil von

• Science (American Association for the Advancement of Science), 2006, Vol.311 (5769), p.1940-1942

Ort / Verlag

American Association for the Advancement of Science

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-{szligbeta}-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-{szligbeta}-D-glucan content in barley grain to a cluster of cellulose synthase-like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-{szligbeta}-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-{szligbeta}-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-{szligbeta}-D-glucan biosynthesis.