Details

Autor(en) / Beteiligte

• Frazar, Christian D
• Orlandi, Palmer A

Titel

Evaluation of Two DNA Template Preparation Methods for Post-Immunomagnetic Separation Detection of Cryptosporidium parvum in Foods and Beverages by PCR

Ist Teil von

• Applied and Environmental Microbiology, 2007-11, Vol.73 (22), p.7474-7476

Ort / Verlag

Washington, DC: American Society for Microbiology

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Cryptosporidium parvum oocysts were recovered by immunomagnetic separation from six artificially contaminated foods. Two DNA isolation methods were subsequently evaluated by PCR. The FTA Concentrator-PS filter provided rapid and reproducible detection, although variability increased at lower inoculum levels (88% and 15% detection in high- and low-inoculum-level samples, respectively). Total DNA extraction generated consistent results at all oocyst levels but resulted in longer analysis time (100% and 59% detection in high- and low-inoculum-level samples, respectively). Also reflected in this study was that the matrix played an important role in the ability to recover oocysts, as sample turbidity, pH, and PCR inhibitors all influenced detection.