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Study on the anthocyanin content of some sour cherry varieties grown in Iasi area, Romania
Ist Teil von
Cercetari agronomice in Moldova (Romania), 2011 (1)
Erscheinungsjahr
2011
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
The purpose of this study is to evaluate the anthocyanin (AC) and phenolic compounds (TPC) content, and the description of anthocyanin profile obtained by HPLC-DAD technique (High-Performance Liquid Chromatography - Diode Array Detector), from hydroalcoholic extracts of four varieties of sour cherry (Prunus cerasus L.) grown in experimental field of Research-Development Station for Fruit growing Iasi, Miroslava area. Were also examined some physical-chemical properties of fruits, variety Mocanesti 16 showing the highest moisture content (87.98%), titratable acidity (1.32 g malic/100g acid) and ascorbic acid (12 mg/100g). AC, determined by pH differential method, had the highest value at Engleze timpurii variety (176.2 ± 0.97 mg/100g) and TPC, determined by the Folin-Ciocalteu colorimetric method, had the maximum value at Mocanesti 16 variety (446.89 ± 0.70 mg GAE/100g). Based on the chromatograms obtained, were identified four anthocyanins: cyanidin (cy)-3- glucoside, cy-3-rutinoside, cy-3-sophoroside, cy-3-glucosylrutinoside, expressed as a percentage of anthocyanins area. Anthocyanin profile obtained was not similar in varieties examined, and the ratio between anthocyanins differed from one variety to another. The data obtained confirm previous results on the sour cherries anthocyanin profile and can be used in food and pharmaceutical industry (functional foods) and as a basis of comparison for future studies.
Scopul acestui studiu este evaluarea continutului in antociani (CA) si compusi fenolici (CFT), precum si descrierea profilului antocianic, obtinut prin tehnica HPLC_DAD (Cromatografie Lichida de Inalta Performanta _ Diode de Detectie), din extractele hidroalcoolice a patru soiuri de visin (Prunus cerasus L.), cultivate in campul experimental al Statiunii de Cercetare-Dezvoltare pentru Pomicultura Iasi, zona Miroslava. Au fost examinate si o serie de proprietati fizico-chimice ale fructelor, soiul Mocanesti 16 prezentand cel mai ridicat continut in umiditate (87,98 %), aciditate titrabila (1,32 g acid malic/100g) si acid ascorbic (12 mg/100g). CA, determinat prin metoda diferentei de pH, a avut valoarea cea mai ridicata la soiul Engleze timpurii (176,2 ± 0,97 mg/100g), iar CFT, determinat prin metoda colorimetrica Folin- Ciocalteu, a avut valoarea maxima la soiul Mocanesti 16 (446,89 ± 0,70 mg GAE/100g). Pe baza cromatogramelor obtinute au fost identificati patru antociani: cianidina (cy)-3-glucozid, cy-3-rutinozid, cy-3-soforozid, cy-3-glucozilrutinozid, exprimati in procente din aria corespunzatoare antocianilor, determinata pentru fiecare soi. Profilul antocianic obtinut nu a fost similar la toate soiurile analizate, iar raportul dintre antociani a variat de la un soi la altul. Valorile obtinute confirma rezultatele anterioare, referitoare la profilul antocianic al visinelor, si vor putea fi utilizate in industria alimentara si farmaceutica (alimente functionale) si ca baza de comparatie pentru studii viitoare.