Details

Autor(en) / Beteiligte

• Seo, M.J. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Im, E.M. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Hur, J.H. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Nam, J.Y. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Hyun, C.G. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea)
• Pyun, Y.R. (Yonsei University, Seoul, Republic of Korea)
• Kim, S.O. (Chem Tech Research Incorporation (C-TRI), Whasung, Republic of Korea), E-mail: sokim@c-tri.com

Titel

Production of Coenzyme Q∧10 by Recombinant E. coli Harboring the Decaprenyl Diphosphate Synthase Gene from Sinorhizobium meliloti

Ist Teil von

• Journal of microbiology and biotechnology, 2006-06, Vol.16 (6)

Erscheinungsjahr

2006

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Decaprenyl diphosphate synthase (DPS) is the key enzyme for the production of coenzyme Q∧10 (CoQ∧10). A dps gene from Sinorhizobium meliloti KCCM 11232 (IFO 14782) was isolated by PCR and then cloned in Escherichia coli. DNA sequencing analysis revealed an open reading frame of 1,017 bp encoding a 338-amino-acid protein. The protein was identical at the 98% level to the putative octaprenyl diphosphate synthase (IspB) of S. meliloti 1021. The deduced amino acid sequence included the DDxxD domains conserved in the majority of the prenyl diphosphate synthases.