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A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and comparedone- and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts.