Details

Autor(en) / Beteiligte

• De, Sachinandan (National Dairy Research Institute, Karnal (India). Animal Biotechnology Centre)
• Yadav, B.R. (National Dairy Research Institute, Karnal (India). Livestock Genome Analysis Laboratory)
• Singh, R.K (Indian Veterinary Research Institute, Izatnagar (India). National Biotechnology Centre)

Titel

Identification of gtg specific microsatellite dna in river buffalo (Bubalus bubalis) genome

Ist Teil von

• Indian journal of animal sciences, 2001-04, Vol.71 (4)

Erscheinungsjahr

2001

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Genomic library of Murrah buffalo was constructed plasmid pBluescript SK II (-) vector. The size of the inserts in the recombinant clone ranged from 0.3-1.0 kb. The primary screening of the genomic library was done to identify the clones carrying insert having (GTG) trinucleotide repeat sequences. Slot blot hybridization of plasmid minipreps helped in selecting the particular clone having more repeats in the insert DNA. The Southern hybridization of linerarized recombinant plasmid DNA could confirm the identify of (GTG) 5 repeats in cloned insert DNA.