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Region-specific changes in 5-HT 1A agonist-induced Extracellular signal-Regulated Kinases 1/2 phosphorylation in rat brain: A quantitative ELISA study
Ist Teil von
Neuropharmacology, 2009, Vol.56 (2), p.350-361
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2009
Quelle
ScienceDirect
Beschreibungen/Notizen
Brain serotonin 5-HT
1A receptor, a traditional target for the treatment of mood disorders, modulates intracellular signalling pathways, such as the Extracellular signal-Regulated Kinases 1/2 (ERK1/2) pathway. The present studies are the first to determine levels of phospho-ERK1/2 (pERK1/2) in brain using a quantitative Enzyme Linked-Immuno-Sorbent Assay.
We examined pERK1/2 levels in rat brain following administration of (+)8-OH-DPAT, buspirone as well as of the more selective, high-efficacy 5-HT
1A agonists F13640 and F13714. Intraperitoneal injection of these compounds increased pERK1/2 in prefrontal cortex and hypothalamus, with a maximum at 5–15
min and a significant effect lasting until 30–60
min post-injection. However, these compounds reduced hippocampal pERK1/2 with a maximum effect at 30
min, persisting until 60
min post-injection. In hippocampus, F13640, F13714 and buspirone inhibited pERK1/2 in a dose-dependent manner as of 0.04, 0.04 and 2.5
mg/kg, respectively. Given these low doses, this response is likely related to activation of sensitive presynaptic 5-HT
1A receptors in the raphe nucleus. 4- and 16-fold higher doses of these compounds were necessary to stimulate pERK1/2 in prefrontal cortex and hypothalamus, respectively,
via direct 5-HT
1A receptor activation. In contrast, (+)8-OH-DPAT was active at similar doses (0.63
mg/kg) in these different regions. Pretreatment with the 5-HT
1A antagonist, WAY100635, completely blocked the effects of these compounds, with the exception of buspirone-induced pERK1/2 increases in hypothalamus.
Thus, 5-HT
1A agonist-induced changes in pERK1/2 in rat brain are time- and dose-dependent and region-specific. Furthermore, F13640, F13714, buspirone, but not (+)8-OH-DPAT, exert their effects
via preferential activation of presynaptic 5-HT
1A receptors.