Details

Autor(en) / Beteiligte

• Charalampidis, Pavlos S.
• Veltsistas, Panos
• Karkabounas, Spyros
• Evangelou, Angelos

Titel

Blue CrO 5 assay: A novel spectrophotometric method for the evaluation of the antioxidant and oxidant capacity of various biological substances

Ist Teil von

• European journal of medicinal chemistry, 2009, Vol.44 (10), p.4162-4168

Ort / Verlag

Elsevier Masson SAS

Erscheinungsjahr

2009

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Oxidative stress plays a pivotal role in the ageing process and in the pathogenesis of numerable diseases. The quantification of the phenomenon is of paramount importance. In the present study, we introduce a novel and simple assay, the Blue CrO 5 assay, for the evaluation of the oxidant and antioxidant capacity of various biological samples and known antioxidants. Chromium peroxide (CrO 5) is produced by ammonium dichromate in an acidic environment in the presence of H 2O 2. It is a deep blue potent oxidant compound, miscible and relatively stable in polar organic solvents, that can be easily measured by spectrophotometry. Its reduction by known antioxidants, both water- and lipid-soluble (ascorbate and α-tocopherol, respectively, in this study), detected spectrophotometrically as a decrease in the absorption and depicted in EPR spectra, can act as a measure of the antioxidant capacity of a certain compound. The assay displays significant sensitivity, stability, linearity, specificity and repeatability. [Display omitted] Chromium peroxide is a deep blue potent oxidant compound miscible and relatively stable in polar organic solvents that can be easily measured by spectrophotometry. Its reduction by known antioxidants, both water- and lipid-soluble (ascorbate and α-tocopherol, respectively, in this study) can act as a measure of the antioxidant capacity of a certain compound.