Details

Autor(en) / Beteiligte

• Greenwood, C.T.
• MacGregor, A.W.
• Ann Milne, E.

Titel

Studies on starch-degrading enzymes : Part II the Z-enzyme from soya beans; purification and properties

Ist Teil von

• Carbohydrate research, 1965, Vol.1 (3), p.229-241

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

1965

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• A method for the isolation and purification of the Z-enzyme in soya beans is described. This procedure involves the formation of the glycogen-enzyme complex. A method for assay of activity is presented. The properties of the purified enzyme have been studies; in particular, the variation of activity and stability with temperature and pH has enabled the nature of the active sites in the enzyme to be investigated. Characterization of the activity of Z-enzyme at pH 3.6, and in the presence of a variety of reagents, has been achieved by viscometric techniques. Both linear and branched glucans are attacked by the purified enzyme. It is concluded that the properties of Z-enzyme are similar to those of other plant α-amylases.