Details

Autor(en) / Beteiligte

• Greenwood, C.T.
• MacGregor, A.W.
• Ann Milne, E.

Titel

Studies on starch-degrading enzymes : Part III. The action pattern of soya-bean z-enzyme

Ist Teil von

• Carbohydrate research, 1965, Vol.1 (4), p.303-311

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

1965

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The action pattern of soya-bean Z-enzyme on linear amylose has been investigated. The kinetics of the initial stages of α-amylolysis have been studied by the use of viscometric techniques, and the process is shown to be an apparently random one. Paper-chromatographic studies of the production of maltodextrins at the “achroic limit” indicated, however, that, at this stage, the attack is non-random. This has been confirmed by ( i) studying the action of the enzyme on individual maltodextrins, and ( ii) measuring quantitatively the yields of dextrins after the “achroic limit”. The latter experimental yields have been compared with those calculated from various theoretical models for the α-amylolytic action. A hypothesis has been developed to account for the observations made at various stages of the α-amylolysis.