Details

Autor(en) / Beteiligte

• Baidukova, Olga
• Kelterborn, Simon
• Sizova, Irina
• Hegemann, Peter

Titel

Chapter 17 - Reverse genetics

Ist Teil von

• The Chlamydomonas Sourcebook, 2023, p.421-430

Auflage

Third Edition

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2023

Beschreibungen/Notizen

• The green alga species Chlamydomonas reinhardtii is a model organism that has not only proven its usefulness for studying plant biology, but also has played a key role in tackling typical mammalian issues connected to cilia and intraflagellar transport. Despite its superior attributes for research, the utility of this organism has been significantly limited due to the difficulties involved in targeted editing of its nuclear genes. Currently, the main available genome-editing tools are zinc-finger nucleases, transcriptional activator-like effector nucleases and—most relevant—the nucleases of the CRISPR-Cas systems. The development of a CRISPR–Cas9 gene-editing system for Chlamydomonas has made the Chlamydomonas genome amenable to targeted and precise gene modifications. This chapter summarizes the recent progress that has been made in the field and discusses points for future improvements in the technique.