Details

Autor(en) / Beteiligte

• Song, Linlin, M.D
• Li, Di, M.D., PhD
• Wang, Juan, M.D
• Meng, Chao, M.D
• Cui, Xiaoguang, M.D., PhD

Titel

Effects of p38 MAPK on lung ischemia-reperfusion injury in diabetic rats

Ist Teil von

• The Journal of surgical research, 2017

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Abstract Background Lung ischemia-reperfusion injury (LIRI) is a pathological process that is observed in several clinical conditions, and p38 mitogen-activated protein kinase (MAPK) is involved. Diabetes mellitus (DM) results in an increased incidence of ischemia-induced organ damage. The aims of this study were to examine the effects of DM on LIRI in a rat model of DM and to explore the possible mechanisms in relation to the p38 MAPK pathway. Method Forty rats were randomly divided into the following five groups (n = 8 each): a control + sham group (CS), a control + IR group (CIR), a DM + sham group (DS), a DM + IR group (DIR), and a DM + IR + SB203580 group (DIRSB). The control and streptozotocin-induced diabetic rats underwent a sham operation or left hilum occlusion for 90 min followed by reperfusion for 4 h. SB203580 was used to inhibit the p38 MAPK pathway. The pulmonary oxygenation index, inflammatory cytokines in the serum, lung edema, histopathology, oxidant stress, apoptosis and phosphorylated/total-p38 MAPK protein levels were measured. Results The DIR group displayed greater concentrations of TNF-α, IL-6 and ICAM-1 and increases in the wet weight-to-dry weight ratio, lung injury scores, malondialdehyde levels, and cellular apoptosis, and these effects were accompanied by lower pulmonary oxygenation compared with the CIR group ( p < 0.05). In the DIR group, the expression levels of p38 MAPK protein were significantly upregulated compared with those of the CIR group. Additionally, all of these alterations were attenuated in the DIRSB group compared with the DIR group. Conclusion Diabetes exacerbates LIRI by activating the p38 MAPK pathway.