Details

Autor(en) / Beteiligte

• Luo, Hou‐Long
• Pi, Jiang
• Zhang, Jun‐Ai
• Yang, En‐Zhuo
• Xu, Huan
• Luo, Hong
• Shen, Ling
• Peng, Ying
• Liu, Gan‐Bin
• Song, Cai‐Mei
• Li, Ke‐Yue
• Wu, Xian‐Jin
• Zheng, Bi‐Ying
• Shen, Hong‐Bo
• Chen, Zheng W
• Xu, Jun‐Fa

Titel

Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA‐874‐3p

Ist Teil von

• Clinical & translational immunology, 2021, Vol.10 (2), p.e1254-n/a

Ort / Verlag

Australia: John Wiley & Sons, Inc

Erscheinungsjahr

2021

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Objectives Genetic and epigenetic mechanisms regulate antimicrobial immunity against Mycobacterium tuberculosis (Mtb) infection. Methods The present study assessed circular RNA TRAPPC6B (circTRAPPC6B) for antimicrobial immune functions and defined mechanisms wherein circTRAPPC6B regulates Mtb growth, autophagy and microRNA in macrophages. Results The Mtb infection of monocytes/macrophages resulted in a significantly decreased level of circTRAPPC6B that inhibited intracellular Mtb growth in macrophages. Conversely, circTRAPPC6B expression enhanced autophagy or autophagy‐associated protein LC3‐II production in Mtb‐infected macrophages. circTRAPPC6B‐enhanced autophagy aggregation or sequestration was also observed in fluorescence in situ hybridisation (FISH) analysis and confocal imaging. Mechanistically, circTRAPPC6B targets an inhibiting element miR‐874‐3p, as shown by bioinformatics, dual‐luciferase reporter gene analysis and pull‐down assay, respectively. Notably, miR‐874‐3p prohibited autophagy via suppressing autophagy protein ATG16L1 by binding to its 3′‐untranslated region (UTR) in Mtb‐infected macrophages and thus promoting intracellular Mtb growth. Concurrently, circTRAPPC6B enhanced autophagy in Mtb‐infected macrophages by blocking the ability of miR‐874‐3p to inhibit ATG16L1. Thus, circTRAPPC6B antagonises the ability of miR‐874‐3p to suppress ATG16L1 expression and activate and enhance autophagy sequestration to restrict Mtb growth in macrophages. Conclusion The current findings suggested that both circTRAPPC6B and miR‐874‐3p mechanisms can be explored as potential therapeutics against Mtb infection. In this study, we found that circTRAPPC6B could inhibit intracellular Mycobacterium tuberculosis (Mtb) growth by inducing autophagy in Mtb‐infected macrophages through abolishing the suppression of miR‐874‐3p on ATG16L1 expression. This study highlights circTRAPPC6B as a potential therapeutic agent for tuberculosis control.