Details

Autor(en) / Beteiligte

• Cunha, Luis Eduardo R.
• Stolet, Adilson A.
• Strauch, Marcelo A.
• Pereira, Victor A.R.
• Dumard, Carlos H.
• Gomes, Andre M.O.
• Monteiro, Fábio L.
• Higa, Luiza M.
• Souza, Patrícia N.C.
• Fonseca, Juliana G.
• Pontes, Francisco E.
• Meirelles, Leonardo G.R.
• Albuquerque, José W.M.
• Sacramento, Carolina Q.
• Fintelman-Rodrigues, Natalia
• Lima, Tulio M.
• Alvim, Renata G.F.
• Marsili, Federico F.
• Caldeira, Marcella Moreira
• Zingali, Russolina B.
• de Oliveira, Guilherme A.P.
• Souza, Thiago M.L.
• Silva, Alexandre S.
• Muller, Rodrigo
• Rodrigues, Daniela del Rosário Flores
• Jesus da Costa, Luciana
• Alves, Arthur Daniel R.
• Pinto, Marcelo Alves
• Oliveira, Andréa C.
• Guedes, Herbert L.M.
• Tanuri, Amilcar
• Castilho, Leda R.
• Silva, Jerson L.

Titel

Polyclonal F(ab’)2 fragments of equine antibodies raised against the spike protein neutralize SARS-CoV-2 variants with high potency

Ist Teil von

• iScience, 2021-11, Vol.24 (11), p.103315-103315, Article 103315

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We used the recombinant trimeric spike (S) glycoprotein in the prefusion conformation to immunize horses for the production of hyperimmune globulins against SARS-CoV-2. Serum antibody titers measured by ELISA were above 1:106, and the neutralizing antibody titer against authentic virus (WT) was 1:14,604 (average PRNT90). Plasma from immunized animals was pepsin digested to remove the Fc portion and purified, yielding an F(ab’)2 preparation with PRNT90 titers 150-fold higher than the neutralizing titers in human convalescent plasma. Challenge studies were carried out in hamsters and showed the in vivo ability of equine F(ab’)2 to reduce viral load in the pulmonary tissues and significant clinical improvement determined by weight gain. The neutralization curve by F(ab’)2 was similar against the WT and P.2 variants, but displaced to higher concentrations by 0.39 log units against the P.1 (Gamma) variant. These results support the possibility of using equine F(ab’)2 preparation for the clinical treatment of COVID patients. [Display omitted] •Trimeric spike glycoprotein induces high level of horse neutralizing antibodies•The F(ab’)2 Ab has neutralizing titer 150-fold higher than human convalescent plasma•The neutralizing activity of F(ab’)2 was high against the P.1 (Gamma) and P.2 variants•Anti-spike F(ab’)2 protected hamsters against SARS-CoV-2 challenge Immunology; Equine immunology; Biological sciences