eLife, 2019-11, Vol.8
2019
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila
- Ist Teil von
- eLife, 2019-11, Vol.8
- Ort / Verlag
- England: eLife Sciences Publications Ltd
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Circadian rhythms are generated by the cyclic transcription, translation, and degradation of clock gene products, including ( ), but how the circadian clock senses and adapts to temperature changes is not completely understood. Here, we show that temperature dramatically changes the splicing pattern of in . We found that at 18°C, TIM levels are low because of the induction of two cold-specific isoforms: and . At 29°C, another isoform, , is upregulated. Isoform switching regulates the levels and activity of TIM as each isoform has a specific function. We found that encodes a protein that rescues the behavioral defects of mutants, and that flies in which is abrogated have abnormal locomotor activity. In addition, miRNA-mediated control limits the expression of some of these isoforms. Finally, data that we obtained using minigenes suggest that alternative splicing might act as a thermometer for the circadian clock.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.44642Titel-ID: cdi_doaj_primary_oai_doaj_org_article_fc681795a82c47918d2640f4d4aa5244
- Format
- –
- Schlagworte
- Adaptation, Physiological - genetics, Alternative Splicing, Animal behavior, Animals, Animals, Genetically Modified, Cell Line, circadian, Circadian Clocks, Circadian Rhythm, Circadian rhythms, Clock gene, Cold, Drosophila, Drosophila melanogaster - cytology, Drosophila melanogaster - genetics, Drosophila melanogaster - metabolism, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Gene Expression Profiling - methods, Insects, Isoforms, Locomotor activity, MicroRNAs - genetics, miRNA, Motor Activity - genetics, Mutants, Neuroscience, Pandas, Physiology, Protein Isoforms - genetics, Protein Isoforms - metabolism, Proteins, RNA, RNA polymerase, splicing, Temperature, timeless, Transcription