Molecules (Basel, Switzerland), 2015-11, Vol.20 (11), p.20699-20708
2015
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- Autor(en) / Beteiligte
- Titel
- Cinidium officinale and its Bioactive Compound, Butylidenephthalide, Inhibit Laser-Induced Choroidal Neovascularization in a Rat Model
- Ist Teil von
- Molecules (Basel, Switzerland), 2015-11, Vol.20 (11), p.20699-20708
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Choroidal neovascularization (CNV) is a common pathology in age-related macular degeneration. In this study, we evaluated in a rat model the effect of an extract of Cinidium officinale Makino and its bioactive compound, butylidenephthalide, on laser-induced CNV. Experimental CNV was induced in Long-Evans rats by laser photocoagulation. C. officinale extract (COE) and butylidenephthalide was intraperitoneally injected once per day for ten days after laser photocoagulation. Choroidal flat mounts were prepared to measure CNV areas and macrophage infiltration. We used a protein array to evaluate the expression levels of angiogenic factors. The CNV area and macrophage infiltration in COE-treated rats were significantly lower than in vehicle-treated rats. COE decreased the expression levels of IGFBP-1, MCP-1, PAI-1, and VEGF. Additionally, butylidenephthalide also inhibited the laser-induced CNV formation and macrophage infiltration and down-regulated the expression of IGFBP-1, MCP-1 and VEGF. These results suggest that COE exerts anti-angiogenic effects on laser-induced CNV by inhibiting the expression of IGFBP-1, MCP-1, and VEGF, indicating that anti-angiogenic activities of COE may be in part due to its bioactive compound, butylidenephthalide.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1420-3049eISSN: 1420-3049DOI: 10.3390/molecules201119728Titel-ID: cdi_doaj_primary_oai_doaj_org_article_fb58f584f5844b6e83822fc8bf446b20
- Format
- –
- Schlagworte
- age-related macular degeneration, Angiogenesis Inhibitors - chemistry, Angiogenesis Inhibitors - pharmacology, Animals, butylidenephthalide, Cell growth, Chemokine CCL2 - genetics, Chemokine CCL2 - metabolism, choroidal neovascularization, Choroidal Neovascularization - drug therapy, Choroidal Neovascularization - etiology, Choroidal Neovascularization - metabolism, Choroidal Neovascularization - pathology, Cinidium officinale, Disease Models, Animal, Gene Expression Regulation - drug effects, Herbal medicine, Insulin-Like Growth Factor Binding Protein 1 - genetics, Insulin-Like Growth Factor Binding Protein 1 - metabolism, Lasers, Lasers - adverse effects, Macrophages - drug effects, Macrophages - metabolism, Macrophages - pathology, Macular degeneration, Phthalic Anhydrides - chemistry, Phthalic Anhydrides - pharmacology, Plant Extracts - chemistry, Plant Extracts - pharmacology, Rats, Retinal detachment, Vascular endothelial growth factor, Vascular Endothelial Growth Factor A - genetics, Vascular Endothelial Growth Factor A - metabolism