Details

Autor(en) / Beteiligte

• Thongsahuan, Sorawat
• Baimai, Visut
• Otsuka, Yasushi
• Saeung, Atiporn
• Tuetun, Benjawan
• Jariyapan, Narissara
• Suwannamit, Sittiporn
• Somboon, Pradya
• Jitpakdi, Atchariya
• Takaoka, Hiroyuki
• Choochote, Wej

Titel

Karyotypic variation and geographic distribution of Anopheles campestris -like (Diptera: Culicidae) in Thailand

Ist Teil von

• Memórias do Instituto Oswaldo Cruz, 2009-07, Vol.104 (4), p.558-566

Ort / Verlag

Brazil: Fundação Oswaldo Cruz, Fiocruz

Erscheinungsjahr

2009

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Seventy-one isolines of Anopheles campestris -like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2 generations, suggesting a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies variation (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.