Details

Autor(en) / Beteiligte

• Yeow, Melissa
• Liu, Fang
• Ma, Rena
• Williams, Timothy J
• Riordan, Stephen M
• Zhang, Li

Titel

Analyses of energy metabolism and stress defence provide insights into Campylobacter concisus growth and pathogenicity

Ist Teil von

• Gut pathogens, 2020-03, Vol.12 (1), p.13-13, Article 13

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2020

Quelle

Springer Online Journals【Remote access available】

Beschreibungen/Notizen

• is an emerging enteric pathogen that is associated with inflammatory bowel disease. Previous studies demonstrated that is non-saccharolytic and hydrogen gas (H ) is a critical factor for growth. In order to understand the molecular basis of the non-saccharolytic and H -dependent nature of growth, in this study we examined the pathways involving energy metabolism and oxidative stress defence in . Bioinformatic analysis of genomes in comparison with the well-studied enteric pathogen was performed. This study found that lacks a number of key enzymes in glycolysis, including glucokinase and phosphofructokinase, and the oxidative pentose phosphate pathway. has an incomplete tricarboxylic acid cycle, with no identifiable succinyl-CoA synthase or fumarate hydratase. was inferred to use fewer amino acids and have fewer candidate substrates as electron donors and acceptors compared to . The addition of DMSO or fumarate to media resulted in significantly increased growth of in the presence of H as an electron donor, demonstrating that both can be used as electron acceptors. Catalase, an essential enzyme for oxidative stress defence in , and various nitrosative stress enzymes, were not found in the genome. Overall, is inferred to have a non-saccharolytic metabolism in which H is central to energy conservation, and a narrow selection of carboxylic acids and amino acids can be utilised as organic substrates. In conclusion, this study provides a molecular basis for the non-saccharolytic and hydrogen-dependent nature of energy metabolism pathways, which provides insights into the growth requirements and pathogenicity of this species.