Details

Autor(en) / Beteiligte

• Linscott, Megan L
• Yildiz, Yoldas
• Flury, Sarah
• Newby, Mikayla L
• Pak, Toni R

Titel

Age and 17β-Estradiol (E 2 ) Facilitate Nuclear Export and Argonaute Loading of microRNAs in the Female Brain

Ist Teil von

• Non-coding RNA, 2023-12, Vol.9 (6), p.74

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Aging in women is accompanied by a dramatic change in circulating sex steroid hormones. Specifically, the primary circulating estrogen, 17β-estradiol (E ), is nearly undetectable in post-menopausal women. This decline is associated with a variety of cognitive and mood disorders, yet hormone replacement therapy is only effective within a narrow window of time surrounding the menopausal transition. Our previous work identified microRNAs as a potential molecular substrate underlying the change in E efficacy associated with menopause in advanced age. Specifically, we showed that E regulated a small subset of mature miRNAs in the aging female brain. In this study, we hypothesized that E regulates the stability of mature miRNAs by altering their subcellular localization and their association with argonaute proteins. We also tested the hypothesis that the RNA binding protein, hnRNP A1, was an important regulator of mature miR-9-5p expression in neuronal cells. Our results demonstrated that E treatment affected miRNA subcellular localization and its association with argonaute proteins differently, depending on the length of time following E deprivation (i.e., ovariectomy). We also provide strong evidence that hnRNP A1 regulates the transcription of pri-miR-9 and likely plays a posttranscriptional role in mature miR-9-5p turnover. Taken together, these data have important implications for considering the optimal timing for hormone replacement therapy, which might be less dependent on age and more related to how long treatment is delayed following menopause.