Asian journal of andrology, 2015-11, Vol.17 (6), p.996-1005
2015
Details
- Autor(en) / Beteiligte
- Titel
- NODAL secreted by male germ cells regulates the proliferation and function of human Sertoli cells from obstructive azoospermia and nonobstructive azoospermia patients
- Ist Teil von
- Asian journal of andrology, 2015-11, Vol.17 (6), p.996-1005
- Ort / Verlag
- China: Medknow Publications and Media Pvt. Ltd
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- This study was designed to explore the regulatory effects of male germ cell secreting factor NODAL on Sertoli cell fate decisions from obstructive azoospermia （OA） and nonobstructive azoospermia （NOA） patients. Human Sertoli cells and male germ cells were isolated using two-step enzymatic digestion and SATPUT from testes of azoospermia patients. Expression of NODAL and its multiple receptors in human Sertoli cells and male germ cells were characterized by reverse transcription-polymerase chain reaction （RT-PCRI and immunochemistry. Human recombinant NODAL and its receptor inhibitor SB431542 were employed to probe their effect on the proliferation of Sertoli cells using the CCK-8 assay. Quantitative PCR and Western blots were utilized to assess the expression of Sertoli cell functional genes and proteins. NODAL was found to be expressed in male germ cells but not in Sertoli cells, whereas its receptors ALK4, ALK7, and ACTR-IIB were detected in Sertoli cells and germ cells, suggesting that NODAL plays a regulatory role in Sertoli cells and germ cells via a paracrine and autocrine pathway, respectively. Human recombinant NODAL could promote the proliferation of human Sertoli cells. The expression of cell cycle regulators, including CYCLIN A, CYCLIN D1 and CYCLIN E, was not remarkably affected by NODAL signaling. NODAL enhanced the expression of essential growth factors, including GDNF, SCF, and BMP4, whereas SB431542 decreased their levels. There was not homogeneity of genes changes by NODAL treatment in Sertoli cells from OA and Sertoli cell-only syndrome （SCO） patients. Collectively, this study demonstrates that NODAL produced by human male germ cells regulates proliferation and numerous gene expression of Sertoli cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1008-682XeISSN: 1745-7262DOI: 10.4103/1008-682X.159722Titel-ID: cdi_doaj_primary_oai_doaj_org_article_f9147d6bf53345e0855ac0b1df2d7f1b
- Format
- –
- Schlagworte
- Activin Receptors, Type I - metabolism, Activin Receptors, Type II - metabolism, Adult, Aspermia, Autocrine Communication, Azoospermia - metabolism, azoospermia, function, human Sertoli cells, NODAL, proliferation, Benzamides - pharmacology, Blotting, Western, Bone Morphogenetic Protein 4 - drug effects, Bone Morphogenetic Protein 4 - metabolism, Cell Proliferation - drug effects, Cyclin A - drug effects, Cyclin A - metabolism, Cyclin D1 - drug effects, Cyclin D1 - metabolism, Cyclin E - drug effects, Cyclin E - metabolism, Development and progression, Dioxoles - pharmacology, Genetic aspects, Germ cells, Glial Cell Line-Derived Neurotrophic Factor - drug effects, Glial Cell Line-Derived Neurotrophic Factor - metabolism, Hogs, Humans, Immunohistochemistry, Infertility, Male, Nodal Protein - metabolism, Nodal Protein - pharmacology, Original, Paracrine Communication, Physiological aspects, Real-Time Polymerase Chain Reaction, Recombinant Proteins - pharmacology, Reverse Transcriptase Polymerase Chain Reaction, Rodents, Sertoli Cells - drug effects, Spermatozoa - secretion, Stem Cell Factor - drug effects, Stem Cell Factor - metabolism, Stem cells, 分泌调节, 患者, 无精子症, 睾丸支持细胞, 细胞增殖, 节点, 阻性, 雄性生殖细胞