Details

Autor(en) / Beteiligte

• Missailidis, Catharina
• Sørensen, Nikolaj
• Ashenafi, Senait
• Amogne, Wondwossen
• Kassa, Endale
• Bekele, Amsalu
• Getachew, Meron
• Gebreselassie, Nebiat
• Aseffa, Abraham
• Aderaye, Getachew
• Andersson, Jan
• Brighenti, Susanna
• Bergman, Peter

Titel

Vitamin D and Phenylbutyrate Supplementation Does Not Modulate Gut Derived Immune Activation in HIV-1

Ist Teil von

• Nutrients, 2019-07, Vol.11 (7), p.1675

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Dysbiosis and a dysregulated gut immune barrier function contributes to chronic immune activation in HIV-1 infection. We investigated if nutritional supplementation with vitamin D and phenylbutyrate could improve gut-derived inflammation, selected microbial metabolites, and composition of the gut microbiota. Treatment-naïve HIV-1-infected individuals ( = 167) were included from a double-blind, randomized, and placebo-controlled trial of daily 5000 IU vitamin D and 500 mg phenylbutyrate for 16 weeks (Clinicaltrials.gov NCT01702974). Baseline and per-protocol plasma samples at week 16 were analysed for soluble CD14, the antimicrobial peptide LL-37, kynurenine/tryptophan-ratio, TMAO, choline, and betaine. Assessment of the gut microbiota involved 16S rRNA gene sequencing of colonic biopsies. Vitamin D + phenylbutyrate treatment significantly increased 25-hydroxyvitamin D levels ( < 0.001) but had no effects on sCD14, the kynurenine/tryptophan-ratio, TMAO, or choline levels. Subgroup-analyses of vitamin D insufficient subjects demonstrated a significant increase of LL-37 in the treatment group ( = 0.02), whereas treatment failed to significantly impact LL-37-levels in multiple regression analysis. Further, no effects on the microbiota was found in number of operational taxonomic units ( = 0.71), Shannon microbial diversity index ( = 0.82), or in principal component analyses ( = 0.83). Nutritional supplementation with vitamin D + phenylbutyrate did not modulate gut-derived inflammatory markers or microbial composition in treatment-naïve HIV-1 individuals with active viral replication.