Details

Autor(en) / Beteiligte

• Sergi, Domenico
• Campbell, Fiona M
• Grant, Christine
• Morris, Amanda C
• Bachmair, Eva-Maria
• Koch, Christiane
• McLean, Fiona H
• Muller, Aifric
• Hoggard, Nigel
• de Roos, Baukje
• Porteiro, Begona
• Boekschoten, Mark V
• McGillicuddy, Fiona C
• Kahn, Darcy
• Nicol, Phyllis
• Benzler, Jonas
• Mayer, Claus-Dieter
• Drew, Janice E
• Roche, Helen M
• Muller, Michael
• Nogueiras, Ruben
• Dieguez, Carlos
• Tups, Alexander
• Williams, Lynda M

Titel

SerpinA3N is a novel hypothalamic gene upregulated by a high-fat diet and leptin in mice

Ist Teil von

• Genes & nutrition, 2018, Vol.13 (1), p.28-28, Article 28

Ort / Verlag

Germany: BioMed Central

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Energy homeostasis is regulated by the hypothalamus but fails when animals are fed a high-fat diet (HFD), and leptin insensitivity and obesity develops. To elucidate the possible mechanisms underlying these effects, a microarray-based transcriptomics approach was used to identify novel genes regulated by HFD and leptin in the mouse hypothalamus. Mouse global array data identified as a novel gene highly upregulated by both a HFD and leptin challenge. In situ hybridisation showed expression upregulation by HFD and leptin in all major hypothalamic nuclei in agreement with transcriptomic gene expression data. Immunohistochemistry and studies in the hypothalamic clonal neuronal cell line, mHypoE-N42 (N42), confirmed that alpha 1-antichymotrypsin (α AC), the protein encoded by , is localised to neurons and revealed that it is secreted into the media. expression in N42 neurons is upregulated by palmitic acid and by leptin, together with and , and all three genes are downregulated by the anti-inflammatory monounsaturated fat, oleic acid. Additionally, palmitate upregulation of in N42 neurons is blocked by the NFκB inhibitor, BAY11, and the upregulation of expression in the hypothalamus by HFD is blunted in IL-1 receptor 1 knockout ( ) mice. These data demonstrate that expression is implicated in nutritionally mediated hypothalamic inflammation.