Details

Autor(en) / Beteiligte

• Sun, Zhifeng
• Lu, Mingmin
• Kim, Woohyun
• Lillehoj, Hyun
• Panebra, Alfredo
• Zhao, Hongyan
• Li, Charles

Titel

Research Note: Development and characterization of monoclonal antibodies specific for chicken interleukin-7 receptor α (CD127)

Ist Teil von

• Poultry science, 2022-10, Vol.101 (10), p.102047-102047, Article 102047

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Chicken recombinant IL-7Ra (chCD127) was expressed and characterized.•Six new mouse monoclonal antibodies (MAbs) specific for chCD127 were developed and characterized.•All mouse anti-CD127 MAbs detected chCD127 protein in ELISA with two clones highly reactive with chicken leukocytes in flow cytometry.•The CD127 as identified by the MAb 3B8 was highly expressed in thymus, lung, and spleen tissues of 3-week-old chickens.•All six anti-chCD127 MAbs inhibited IL-7-induced thymocyte proliferation. CD127, also named interleukin-7 receptor (IL-7R), is expressed on various cell types including naive and memory T cells, and plays a critical role in the differentiation and activation of T lymphocytes. The availability of poultry-specific immune reagents to identify and measure chicken CD127 response will enhance fundamental and applied research in poultry immunology. Mouse monoclonal antibodies (MAbs) against chicken CD127 (chCD127) were developed and characterized. More specifically, a 678 bp ectodomain of chCD127 gene was cloned in the pET28a (+) vector and expressed in BL21-AI E. coli competent cells. The recombinant chCD127 protein with a size of 30 KDa which was also recognized by a mouse anti-human CD127 MAb (Clone G-11) was used to immunize mice, and 6 new mouse MAbs which specifically detected chicken CD127 were developed and characterized. Availability of these new sets of chCD127-specific MAbs will facilitate the immunological studies on CD127 in poultry, especially in understanding effector and memory T immune cell responses in normal and diseased states.