Details

Autor(en) / Beteiligte

• Tomsík, Pavel
• Stoklasová, Alena
• Micuda, Stanislav
• Niang, Mohamed
• Suba, Petr
• Knízek, Jirí
• Rezácová, Martina

Titel

Evaluation of the antineoplastic activity of L-rhamnose in vitro. A comparison with 2-deoxyglucose

Ist Teil von

• Acta medica Lékarskí fakulty Univerzity Karlovy v Hradci Králove, 2008, Vol.51 (2), p.113-119

Ort / Verlag

Czech Republic: Karolinum Press

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• The effect of unsubstituted deoxyhexoses, 2-deoxy-D-glucose (2-DG) and L-fucose, on tumor cells has been reported in several papers throughout the last decades. That of a similar deoxysugar, L-rhamnose, which is synthesized in bacteria and plants but not in animal cells, has until today not been explored. In the present study, we examined the effect of L-rhamnose on DNA and protein synthesis, growth and the potential induction of apoptosis of tumor cells in vitro. Using 2-DG for comparison, we studied the effect of L-rhamnose in concentrations up to 20 (32 resp.) mmol/l on the initial velocity of the incorporation of labeled precursors of DNA and proteins in short term cultures of both mouse Ehrlich ascites tumor (EAT) and human HL-60 cells in vitro, and further, on cell proliferation and apoptosis induction in HL-60 cells. Neither cytotoxic nor cytostatic effects of L-rhamnose were observed with the exception of slightly pronounced inhibition of DNA synthesis in EAT cells. From the lacking inhibition of the protein synthesis it can be considered that L-rhamnose does not interfere with energy metabolism, at least not in a similar manner as 2-DG.