Nature communications, 2021-02, Vol.12 (1), p.741-741, Article 741
2021
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- Autor(en) / Beteiligte
- Titel
- Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution
- Ist Teil von
- Nature communications, 2021-02, Vol.12 (1), p.741-741, Article 741
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2021
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The heterogeneous nature of tumour microenvironment (TME) underlying diverse treatment responses remains unclear in nasopharyngeal carcinoma (NPC). Here, we profile 176,447 cells from 10 NPC tumour-blood pairs, using single-cell transcriptome coupled with T cell receptor sequencing. Our analyses reveal 53 cell subtypes, including tumour-infiltrating CD8 T, regulatory T (Treg), and dendritic cells (DCs), as well as malignant cells with different Epstein-Barr virus infection status. Trajectory analyses reveal exhausted CD8 T and immune-suppressive TNFRSF4 Treg cells in tumours might derive from peripheral CX3CR1 CD8 T and naïve Treg cells, respectively. Moreover, we identify immune-regulatory and tolerogenic LAMP3 DCs. Noteworthily, we observe intensive inter-cell interactions among LAMP3 DCs, Treg, exhausted CD8 T, and malignant cells, suggesting potential cross-talks to foster an immune-suppressive niche for the TME. Collectively, our study uncovers the heterogeneity and interacting molecules of the TME in NPC at single-cell resolution, which provide insights into the mechanisms underlying NPC progression and the development of precise therapies for NPC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-021-21043-4Titel-ID: cdi_doaj_primary_oai_doaj_org_article_f1978be2a763451db9da5aca4be70f2e
- Format
- –
- Schlagworte
- Cancer, CD8 antigen, CD8-Positive T-Lymphocytes - enzymology, CD8-Positive T-Lymphocytes - metabolism, Cell interactions, Cells, Cultured, CX3CR1 protein, Dendritic cells, Dendritic Cells - immunology, Dendritic Cells - metabolism, Epstein-Barr virus, Gene expression, Head and Neck Neoplasms - immunology, Head and Neck Neoplasms - metabolism, Heterogeneity, High-Throughput Nucleotide Sequencing, Humans, Lymphocytes, Lymphocytes T, Male, Microenvironments, Nasopharyngeal carcinoma, Nasopharyngeal Carcinoma - immunology, Nasopharyngeal Carcinoma - metabolism, T cell receptors, T-Lymphocytes, Regulatory - immunology, T-Lymphocytes, Regulatory - metabolism, Throat cancer, Trajectory analysis, Transcriptomes, Tumor microenvironment, Tumor Microenvironment - immunology, Tumor Microenvironment - physiology, Tumors