Details

Autor(en) / Beteiligte

• Fradj, Narimene
• Gonçalves Dos Santos, Karen Cristine
• de Montigny, Nicolas
• Awwad, Fatima
• Boumghar, Yacine
• Germain, Hugo
• Desgagné-Penix, Isabel

Titel

RNA-Seq de Novo Assembly and Differential Transcriptome Analysis of Chaga ( Inonotus obliquus ) Cultured with Different Betulin Sources and the Regulation of Genes Involved in Terpenoid Biosynthesis

Ist Teil von

• International journal of molecular sciences, 2019-09, Vol.20 (18), p.4334

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2019

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Chaga ( ) is a medicinal fungus used in traditional medicine of Native American and North Eurasian cultures. Several studies have demonstrated the medicinal properties of chaga's bioactive molecules. For example, several terpenoids (e.g., betulin, betulinic acid and inotodiol) isolated from cells have proven effectiveness in treating different types of tumor cells. However, the molecular mechanisms and regulation underlying the biosynthesis of chaga terpenoids remain unknown. In this study, we report on the optimization of growing conditions for cultured in presence of different betulin sources (e.g., betulin or white birch bark). It was found that better results were obtained for a liquid culture pH 6.2 at 28 °C. In addition, a de novo assembly and characterization of transcriptome in these growth conditions using Illumina technology was performed. A total of 219,288,500 clean reads were generated, allowing for the identification of 20,072 transcripts of including transcripts involved in terpenoid biosynthesis. The differential expression of these genes was confirmed by quantitative-PCR. This study provides new insights on the molecular mechanisms and regulation of terpenoid production. It also contributes useful molecular resources for gene prediction or the development of biotechnologies for the alternative production of terpenoids.