Details

Autor(en) / Beteiligte

• Gentilini, María Virginia
• Pesce Viglietti, Ayelén Ivana
• Arriola Benitez, Paula Constanza
• Iglesias Molli, Andrea Elena
• Cerrone, Gloria Edith
• Giambartolomei, Guillermo Hernán
• Delpino, María Victoria

Titel

Inhibition of Osteoblast Function by Brucella abortus is Reversed by Dehydroepiandrosterone and Involves ERK1/2 and Estrogen Receptor

Ist Teil von

• Frontiers in immunology, 2018-01, Vol.9, p.88-88

Ort / Verlag

Switzerland: Frontiers Research Foundation

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek

Beschreibungen/Notizen

• induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans, and we have previously demonstrated that infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during infection. infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis, and reversing the inhibitory effect of on osteoblast differentiation and function. By contrast, cortisol increased the effect of infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). infection inhibited GRα expression. Cell responses to cortisol not only depend on GR expression but also on its intracellular bioavailability, that is, dependent on the activity of the isoenzymes 11β-hydroxysteroid dehydrogenase (HSD) type-1, 11β-HSD2 (which convert cortisone to cortisol and , respectively). Alterations in the expression of these isoenzymes in bone cells are associated with bone loss. infection increased 11β-HSD1 expression but had no effect on 11β-HSD2. DHEA reversed the inhibitory effect induced by infection on osteoblast matrix deposition in an estrogen receptor- and ERK1/2-dependent manner. We conclude that DHEA intervention improves osteoblast function during infection making it a potential candidate to ameliorate the osteoarticular symptoms of brucellosis.