Nature communications, 2022-02, Vol.13 (1), p.652-13, Article 652
2022
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- Autor(en) / Beteiligte
- Titel
- Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening
- Ist Teil von
- Nature communications, 2022-02, Vol.13 (1), p.652-13, Article 652
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2022
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Stomatal opening requires the provision of energy in the form of ATP for proton pumping across the guard cell (GC) plasma membrane and for associated metabolic rearrangements. The source of ATP for GCs is a matter of ongoing debate that is mainly fuelled by controversies around the ability of GC chloroplasts (GCCs) to perform photosynthesis. By imaging compartment-specific fluorescent ATP and NADPH sensor proteins in Arabidopsis, we show that GC photosynthesis is limited and mitochondria are the main source of ATP. Unlike mature mesophyll cell (MC) chloroplasts, which are impermeable to cytosolic ATP, GCCs import cytosolic ATP through NUCLEOTIDE TRANSPORTER (NTT) proteins. GCs from ntt mutants exhibit impaired abilities for starch biosynthesis and stomatal opening. Our work shows that GCs obtain ATP and carbohydrates via different routes from MCs, likely to compensate for the lower chlorophyll contents and limited photosynthesis of GCCs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-022-28263-2Titel-ID: cdi_doaj_primary_oai_doaj_org_article_f06e57474de843cf9eb20db051423788
- Format
- –
- Schlagworte
- Adenosine triphosphate, Adenosine Triphosphate - metabolism, Arabidopsis, Arabidopsis - cytology, Arabidopsis - genetics, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, ATP, Biological Transport, Biosynthesis, Carbohydrates, Chlorophyll, Chloroplasts, Chloroplasts - drug effects, Chloroplasts - metabolism, Chloroplasts - radiation effects, Cytosol - metabolism, Fluorescence, Guard cells, Hydrogen Peroxide - pharmacology, Imports, Light, Mesophyll, Mesophyll Cells - cytology, Mesophyll Cells - metabolism, Mesophyll Cells - radiation effects, Microscopy, Confocal, Mitochondria, NADP - metabolism, Nucleotide Transport Proteins - genetics, Nucleotide Transport Proteins - metabolism, Nucleotides, Oxidants - pharmacology, Photosynthesis, Plant Epidermis - cytology, Plant Epidermis - metabolism, Plant Leaves - cytology, Plant Leaves - metabolism, Plant Stomata - cytology, Plant Stomata - metabolism, Plant Stomata - physiology, Plants, Genetically Modified, Protein transport, Proteins, Starch, Starch - metabolism, Stomata