Details

Autor(en) / Beteiligte

• Steranka, Jared P
• Tang, Zuojian
• Grivainis, Mark
• Huang, Cheng Ran Lisa
• Payer, Lindsay M
• Rego, Fernanda O R
• Miller, Thiago Luiz Araujo
• Galante, Pedro A F
• Ramaswami, Sitharam
• Heguy, Adriana
• Fenyö, David
• Boeke, Jef D
• Burns, Kathleen H

Titel

Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome

Ist Teil von

• Mobile DNA, 2019-03, Vol.10 (1), p.8-8, Article 8

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Transposable elements make up a significant portion of the human genome. Accurately locating these mobile DNAs is vital to understand their role as a source of structural variation and somatic mutation. To this end, laboratories have developed strategies to selectively amplify or otherwise enrich transposable element insertion sites in genomic DNA. Here we describe a technique, Transposon Insertion Profiling by sequencing (TIPseq), to map Long INterspersed Element 1 (LINE-1, L1) retrotransposon insertions in the human genome. This method uses vectorette PCR to amplify species-specific L1 (L1PA1) insertion sites followed by paired-end Illumina sequencing. In addition to providing a step-by-step molecular biology protocol, we offer users a guide to our pipeline for data analysis, TIPseqHunter. Our recent studies in pancreatic and ovarian cancer demonstrate the ability of TIPseq to identify invariant (fixed), polymorphic (inherited variants), as well as somatically-acquired L1 insertions that distinguish cancer genomes from a patient's constitutional make-up. TIPseq provides an approach for amplifying evolutionarily young, active transposable element insertion sites from genomic DNA. Our rationale and variations on this protocol may be useful to those mapping L1 and other mobile elements in complex genomes.