Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Ergebnis 1 von 268
International journal of molecular sciences, 2021-07, Vol.22 (13), p.7124
2021
Volltextzugriff (PDF)

Details

Autor(en) / Beteiligte
Titel
Single Copy Oligonucleotide Fluorescence In Situ Hybridization Probe Design Platforms: Development, Application and Evaluation
Ist Teil von
  • International journal of molecular sciences, 2021-07, Vol.22 (13), p.7124
Ort / Verlag
Basel: MDPI AG
Erscheinungsjahr
2021
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
  • Oligonucleotides fluorescence in situ hybridization (Oligo-FISH) is an emerging technology and is an important tool in research areas such as detection of chromosome variation, identification of allopolyploid, and deciphering of three-dimensional (3D) genome structures. Based on the demand for highly efficient oligo probes for oligo-FISH experiments, increasing numbers of tools have been developed for probe design in recent years. Obsolete oligonucleotide design tools have been adapted for oligo-FISH probe design because of their similar considerations. With the development of DNA sequencing and large-scale synthesis, novel tools have been designed to increase the specificity of designed oligo probes and enable genome-scale oligo probe design, which has greatly improved the application of single copy oligo-FISH. Despite this, few studies have introduced the development of the oligo-FISH probe design tools and their application in FISH experiments systematically. Besides, a comprehensive comparison and evaluation is lacking for the available tools. In this review, we provide an overview of the oligo-FISH probe design process, summarize the development and application of the available tools, evaluate several state-of-art tools, and eventually provide guidance for single copy oligo-FISH probe design.
Sprache
Englisch
Identifikatoren
ISSN: 1422-0067, 1661-6596
eISSN: 1422-0067
DOI: 10.3390/ijms22137124
Titel-ID: cdi_doaj_primary_oai_doaj_org_article_ef3c70b05204474a9136d3f685bf49c3

Weiterführende Literatur

Empfehlungen zum selben Thema automatisch vorgeschlagen von bX