Details

Autor(en) / Beteiligte

• Matern, Maggie S.
• Durruthy-Durruthy, Robert
• Birol, Onur
• Darmanis, Spyros
• Scheibinger, Mirko
• Groves, Andrew K.
• Heller, Stefan

Titel

Transcriptional dynamics of delaminating neuroblasts in the mouse otic vesicle

Ist Teil von

• Cell reports (Cambridge), 2023-06, Vol.42 (6), p.112545-112545, Article 112545

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• An abundance of research has recently highlighted the susceptibility of cochleovestibular ganglion (CVG) neurons to noise damage and aging in the adult cochlea, resulting in hearing deficits. Furthering our understanding of the transcriptional cascades that contribute to CVG development may provide insight into how these cells can be regenerated to treat inner ear dysfunction. Here we perform a high-depth single-cell RNA sequencing analysis of the E10.5 otic vesicle and its surrounding tissues, including CVG precursor neuroblasts and emerging CVG neurons. Clustering and trajectory analysis of otic-lineage cells reveals otic markers and the changes in gene expression that occur from neuroblast delamination toward the development of the CVG. This dataset provides a valuable resource for further identifying the mechanisms associated with CVG development from neurosensory competent cells within the otic vesicle. [Display omitted] •scRNA-seq of micro-dissected otic vesicles identifies three otic populations at E10.5•Otic neuroblasts express early neuronal, EMT, and proliferation markers•Expression dynamics analysis suggests potential regulators of neuroblast delamination•Integration with published E9.5 and E11.5 data results in a comprehensive resource Matern et al. provide a validated resource of E10.5 otic vesicle, neuroblast, and early otic neuron single-cell profiles. The neuroblast stage is defined by early neuronal and proliferative genes. Integrating published otic-lineage data from bracketing time points indicates neuroblast delamination as a dynamic process.