Details

Autor(en) / Beteiligte

• Zang, Liqing
• Shimada, Yasuhito
• Miyake, Hideto
• Nishimura, Norihiro

Titel

Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos

Ist Teil von

• Ecotoxicology and environmental safety, 2022-02, Vol.231, p.113211-113211, Article 113211

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Ultraviolet (UV) rays can be both harmful and beneficial to humans. This study aimed to investigate the toxicity and safety of ultraviolet C (UVC) exposure in living organisms and the corresponding biodefense molecular mechanisms. Zebrafish embryos, at an early developmental stage (5–6 h post-fertilization), were irradiated with increasing UVC dosages using high-efficiency deep-ultraviolet light-emitting diodes (278 nm). Morphological phenotypes including survival rate, hatching rate, heart rate, and malformation rate were evaluated. Compared to un-irradiated controls, all zebrafish embryos exposed to 4.5 mJ/cm2 UVC survived and showed no significant difference in hatching and heart rate. However, 7.5 mJ/cm2 of UVC irradiation caused a significantly decreased survival rate (37.5%) and an increased malformation rate (81.8%). Therefore, 4.5 mJ/cm2 was chosen as the limit dosage that the internal biodefense system of zebrafish embryos can protect against UVC radiation. Transcriptome analysis (RNA sequencing) performed on 3 min and 3 days post-irradiation embryos (4.5 mJ/cm2) revealed the molecular mechanisms underlying the response of zebrafish embryos to irradiation. The embryos quickly responded to UVC-induced stress by activating the p53 signaling pathway. In addition, after 3 days of recuperation, the embryos showed activation of signal transducer and activator of transcription (STAT) signaling pathway. To our knowledge, this is the first study to evaluate the toxicological effects and the molecular mechanism of biodefense in zebrafish embryos upon 278 nm UVC irradiation. [Display omitted] •The toxicity and safety of UVC irradiation in living organisms were investigated.•Zebrafish embryos at the early developmental stage (5–6 h post-fertilization) survived after 4.5 mJ/cm2 of UVC irradiation.•Zebrafish embryos respond quickly to UVC-induced stress via activated p53 signaling pathway.•Zebrafish embryos showed activated STAT signaling pathway after three days of recovery from UVC irradiation.