Details

Autor(en) / Beteiligte

• Balmert, Stephen C.
• Ghozloujeh, Zohreh Gholizadeh
• Carey, Cara Donahue
• Williams, Li’an H.
• Zhang, Jiying
• Shahi, Preeti
• Amer, Maher
• Sumpter, Tina L.
• Erdos, Geza
• Korkmaz, Emrullah
• Falo, Louis D.

Titel

A microarray patch SARS-CoV-2 vaccine induces sustained antibody responses and polyfunctional cellular immunity

Ist Teil von

• iScience, 2022-10, Vol.25 (10), p.105045, Article 105045

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Sustainable global immunization campaigns against COVID-19 and other emerging infectious diseases require effective, broadly deployable vaccines. Here, we report a dissolvable microarray patch (MAP) SARS-CoV-2 vaccine that targets the immunoresponsive skin microenvironment, enabling efficacious needle-free immunization. Multicomponent MAPs delivering both SARS-CoV-2 S1 subunit antigen and the TLR3 agonist Poly(I:C) induce robust antibody and cellular immune responses systemically and in the respiratory mucosa. MAP vaccine-induced antibodies bind S1 and the SARS-CoV-2 receptor-binding domain, efficiently neutralize the virus, and persist at high levels for more than a year. The MAP platform reduces systemic toxicity of the delivered adjuvant and maintains vaccine stability without refrigeration. When applied to human skin, MAP vaccines activate skin-derived migratory antigen-presenting cells, supporting the feasibility of human translation. Ultimately, this shelf-stable MAP vaccine improves immunogenicity and safety compared to traditional intramuscular vaccines and offers an attractive alternative for global immunization efforts against a range of infectious pathogens. [Display omitted] •An immunogenic, safe, shelf-stable microarray patch (MAP) SARS-CoV-2 vaccine•Polyfunctional cellular immunity and long-lasting antibody responses•Robust respiratory tract immunity via skin vaccination•Translational immune correlates of MAP SARS-CoV-2 vaccine in human skin Immunology; Virology; Medical biotechnology