Details

Autor(en) / Beteiligte

• Razakandrainibe, Romy
• Mérat, Célia
• Kapel, Nathalie
• Sautour, Marc
• Guyot, Karine
• Gargala, Gilles
• Ballet, Jean-Jacques
• Le Pape, Patrice
• Dalle, Frédéric
• Favennec, Loïc

Titel

Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples

Ist Teil von

• Microorganisms (Basel), 2021-01, Vol.9 (2), p.209

Ort / Verlag

Switzerland: MDPI

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA kit, Savyon Diagnostics, Israel) for the detection of spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.