Details

Autor(en) / Beteiligte

• Nolan, James K.
• Nguyen, Tran N.H.
• Fattah, Mara
• Page, Jessica C.
• Shi, Riyi
• Lee, Hyowon

Titel

Ex vivo electrochemical measurement of glutamate release during spinal cord injury

Ist Teil von

• MethodsX, 2019-01, Vol.6, p.1894-1900

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• [Display omitted] Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.