Details

Autor(en) / Beteiligte

• Cassidy, Noah A.J.
• Fish, Carolyn S.
• Levy, Claire N.
• Roychoudhury, Pavitra
• Reeves, Daniel B.
• Hughes, Sean M.
• Schiffer, Joshua T.
• Benki-Nugent, Sarah
• John-Stewart, Grace
• Wamalwa, Dalton
• Jerome, Keith R.
• Overbaugh, Julie
• Hladik, Florian
• Lehman, Dara A.

Titel

HIV reservoir quantification using cross-subtype multiplex ddPCR

Ist Teil von

• iScience, 2022-01, Vol.25 (1), p.103615-103615, Article 103615

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A major barrier to conducting HIV cure research in populations with the highest HIV burden is the lack of an accurate assay to quantify the replication-competent reservoir across the dominant global HIV-1 subtypes. Here, we modify a subtype B HIV-1 assay that quantifies both intact and defective proviral DNA, adapting it to accommodate cross-subtype HIV-1 sequence diversity. We show that the cross-subtype assay works on subtypes A, B, C, D, and CRF01_AE and can detect a single copy of intact provirus. In longitudinal blood samples from Kenyan infants infected with subtypes A and D, patterns of intact and total HIV DNA follow the decay of plasma viral load over time during antiretroviral therapy, with intact HIV DNA comprising 7% (range 1%–33%) of the total HIV DNA during HIV RNA suppression. This high-throughput cross-subtype reservoir assay will be useful in HIV cure research in Africa and Asia, where HIV prevalence is highest. [Display omitted] •High-throughput HIV reservoir assays for diverse HIV subtypes are needed•Cross-subtype intact proviral DNA assay (CS-IPDA) works across global HIV-1 subtypes•CS-IPDA can be used in cure studies in Africa and Asia where HIV prevalence is high•CS-IPDA on samples from Kenyan infants on ART show expected decay of intact HIV DNA Virology; Genotyping