Details

Autor(en) / Beteiligte

• Leong, Yang Sing
• Mokhtar, Mohd Hadri Hafiz
• Ahmad Nazri, Nur Afifah
• Abu Bakar, Mohd Hafiz
• Zan, Mohd Saiful Dzulkefly
• Arsad, Norhana
• Abdul, Peer Mohamed
• A. Bakar, Ahmad Ashrif

Titel

Discriminative spatial localisation in confocal reflectance and carbon dots-based fluorescence imaging using mixed-mode endoscopic scanner

Ist Teil von

• International journal of optomechatronics, 2024-12, Vol.18 (1)

Ort / Verlag

Taylor & Francis Group

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Taylor & Francis Journals Auto-Holdings Collection

Beschreibungen/Notizen

• AbstractCarbon dots (CDs)-based fluorescence imaging with confocal reflectance effectively localises labelled structures with clear sample visualization. Yet, integrating these modalities into single-fibre cantilever-based endoscopic scanners poses challenges. Thus, we present an endoscopic scanner with a mixed-mode fibre cantilever for dual modalities imaging. The cantilever features two-attached fibre construction for multiple illumination and detection channels without additional optical components. The measured lateral and axial resolutions are 5.81 and 6.52 µm, respectively with field-of-view of 408 × 488 µm. The fluorescence detection capability was first verified using fluorescent microspheres and CD-embedded polymer film, followed by confocal reflectance and CDs-based fluorescence imaging on labelled fibrous network and plant samples. The results demonstrate the complementary strengths of confocal reflectance, which extracts surface profiles, and CDs-based fluorescence, which captures fluorescence signals, leading to precise localization of fluorescence signals within the sample. Switching modality schemes at the scanner’s back-end enables implementation of different modalities.