Biomolecules (Basel, Switzerland), 2024-09, Vol.14 (10), p.1206
2024
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- Autor(en) / Beteiligte
- Titel
- Impact of SDF-1 and AMD3100 on Hair Follicle Dynamics in a Chronic Stress Model
- Ist Teil von
- Biomolecules (Basel, Switzerland), 2024-09, Vol.14 (10), p.1206
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2024
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Chronic stress is a common cause of hair loss, involving inflammatory responses and changes in cellular signaling pathways. This study explores the mechanism of action of the SDF-1/CXCR4 signaling axis in chronic stress-induced hair loss. The research indicates that SDF-1 promotes hair follicle growth through the PI3K/Akt and JAK/STAT signaling pathways. Transcriptome sequencing analysis was conducted to identify differentially expressed genes in the skin of normal and stressed mice, with key genes SDF-1/CXCR4 selected through machine learning and a protein-protein interaction network established. A chronic stress mouse model was created, with injections of SDF-1 and AMD3100 administered to observe hair growth, weight changes, and behavioral alterations and validate hair follicle activity. Skin SDF-1 concentrations were measured, differentially expressed genes were screened, and pathways were enriched. Activation of the PI3K/Akt and JAK/STAT signaling pathways was assessed, and siRNA technology was used in vitro to inhibit the expression of SDF-1 or CXCR4. SDF-1 promoted hair follicle activity, with the combined injection of SDF-1 and AMD3100 weakening this effect. The activation of the PI3K/Akt and JAK/STAT signaling pathways was observed in the SDF-1 injection group, confirmed by Western blot and immunofluorescence. Silencing SDF-1 through siRNA-mediated inhibition reduced cell proliferation and migration abilities. SDF-1 promotes hair growth in chronic stress mice by activating the PI3K/Akt and JAK/STAT pathways, an effect reversible by AMD3100. The SDF-1/CXCR4 axis may serve as a potential therapeutic target for stress-induced hair loss.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2218-273XeISSN: 2218-273XDOI: 10.3390/biom14101206Titel-ID: cdi_doaj_primary_oai_doaj_org_article_e6722e222d21495ea7aa0c236110d895
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, AKT protein, Alopecia - drug therapy, Alopecia - metabolism, Anesthesia, Animals, Behavior, Benzylamines - pharmacology, Cell migration, Cell proliferation, Chemokine CXCL12 - genetics, Chemokine CXCL12 - metabolism, chronic stress, CXCR4, CXCR4 protein, Cyclams - pharmacology, Disease Models, Animal, Experiments, Follicles, Food, Genes, Hair Follicle - drug effects, Hair Follicle - metabolism, Hair loss, Immunofluorescence, JAK/STAT pathway, Machine learning, Male, Mice, Mice, Inbred C57BL, Phosphatidylinositol 3-Kinases - metabolism, PI3K/Akt pathway, Protein interaction, Protein-protein interactions, Proto-Oncogene Proteins c-akt - metabolism, Quality of life, Receptors, CXCR4 - genetics, Receptors, CXCR4 - metabolism, SDF-1, SDF-1 protein, Sequence analysis, Signal transduction, Signal Transduction - drug effects, siRNA, Skin, Stress, Stress (Psychology), Swimming, Therapeutic targets, Transcription activation, Transcriptomes