Toxins, 2024-04, Vol.16 (4), p.194
2024
Details
- Autor(en) / Beteiligte
- Titel
- Sortase-Modified Cholera Toxoids Show Specific Golgi Localization
- Ist Teil von
- Toxins, 2024-04, Vol.16 (4), p.194
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2024
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase labeling approach to generate site-specific N-terminally modified variants of both the A2-B heterohexamer and B pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B pentamer showed an unexpectedly specific localization in the medial/trans-Golgi. This study suggests a future role for specifically labeled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labeling of lipid rafts in fixed cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2072-6651eISSN: 2072-6651DOI: 10.3390/toxins16040194Titel-ID: cdi_doaj_primary_oai_doaj_org_article_e598648757f8438bb29befaeb6e4b709
- Format
- –
- Schlagworte
- Aminoacyltransferases - genetics, Aminoacyltransferases - metabolism, Animals, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Biological products, Biology, Cells, cellular imaging, Cholera, cholera toxin, Cholera Toxin - metabolism, Chromatography, Cysteine Endopeptidases - metabolism, Cytology, Endocytosis, Enzymes, Gangliosides, Golgi apparatus, Golgi Apparatus - metabolism, Golgi body, Gram-positive bacteria, Humans, Labeling, Labelling, Lipid rafts, Lipids, Localization, Mammalian cells, Peptides, Polysaccharides, Protein binding, protein labeling, Proteins, Rafting, Sortase, Toxins, Toxoids, Tracing, Waterborne diseases