Asian Pacific journal of tropical biomedicine, 2015-12, Vol.5 (12), p.987-991
2015
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Details
- Autor(en) / Beteiligte
- Titel
- Deletion of Salmonella enterica serovar typhimurium sipC gene
- Ist Teil von
- Asian Pacific journal of tropical biomedicine, 2015-12, Vol.5 (12), p.987-991
- Ort / Verlag
- Elsevier B.V
- Erscheinungsjahr
- 2015
- Quelle
- ScienceDirect
- Beschreibungen/Notizen
- Objective:To construct a novel plasmid as Salmonella enterica serovar typhimurium(S.typhimurium)sip C gene knockouts candidate.Methods:In this research,50upstream and 30downstream regions of S.typhimurium sip C gene and kanamycin gene were PCR amplified.Each of these DNA fragment was cloned into p GEM T-easy vector.The construct was confirmed by PCR and restriction digest.Results:PCR amplified 320,206 and 835 bp DNA fragments were subcloned into p ET-32 vector resulting with a plasmid called p ET-32-sip C up-kan-sip C down.Conclusions:The new plasmid(p ET-32-sip C up-kan-sip C down)is useful for genetic engineering and for future manipulation of S.typhimurium sip C gene.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2221-1691eISSN: 2588-9222DOI: 10.1016/j.apjtb.2015.09.003Titel-ID: cdi_doaj_primary_oai_doaj_org_article_e54f31aad37645ddae310c0efeba7435
- Format
- –
- Schlagworte
- enterica, gene, Gene construct, pET-32 vector, Salmonella, Salmonella enterica serovar typhimurium, serovar, typhimurium, sip, sipC gene, TA cloning