International journal of molecular sciences, 2020-04, Vol.21 (8), p.2785
2020
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- Autor(en) / Beteiligte
- Titel
- Chondrogenic Potential of Pellet Culture Compared to High-Density Culture on a Bacterial Cellulose Hydrogel
- Ist Teil von
- International journal of molecular sciences, 2020-04, Vol.21 (8), p.2785
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2020
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Cell-based approaches of cartilage lesions use different culture systems to obtain optimal cell quality. Pellet cultures with high cellular density (HD) are the gold standard to keep chondrocytes in a differentiated stage. Bacterial cellulose (BC) hydrogel is discussed to prevent cellular aging and dedifferentiation. The hypothesis of this study was that HD culture on BC hydrogel (HD hydrogel) might reach the chondrogenic potential of pellet culture (pellet). Human articular osteoarthritic (OA) and non-osteoarthritic (non-OA) chondrocytes were cultured for seven days within pellets and compared to HD hydrogel and HD polystyrene. Gene expression analysis and histological assessment were performed. We observed no significant change of expression by the culture system (pellet, HD hydrogel and HD polystyrene) but a significant change of -ratio, with the highest ratio in pellets. Chondrocytes on HD hydrogel showed an elevated expression of and on polystyrene an increased expression of and . The patterns of gene expression changes observed in OA and non-OA chondrocytes in reaction to the different culture systems were similar in those two cell groups. Pellet cultures moreover formed a histomorphologically superior neocartilage. Concluding, human chondrocytes kept the potential to express in all HD culture systems. However, pellets excelled in a higher -ratio, a higher extracellular matrix deposit and in not developing degeneration and dedifferentiation markers. This underlines the superiority of pellet culture in maintaining the chondrogenic potential of human chondrocytes in vitro.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1422-0067, 1661-6596eISSN: 1422-0067DOI: 10.3390/ijms21082785Titel-ID: cdi_doaj_primary_oai_doaj_org_article_e46d427f73204b2ba270e664673c4b37
- Format
- –
- Schlagworte
- Aggrecans - genetics, Aggrecans - metabolism, Aging, bacterial cellulose hydrogel, Biomedical materials, Cartilage, Cartilage diseases, cartilage repair, Cell culture, Cell Culture Techniques - methods, Cells, Cultured, Cellulose, Cellulose - chemistry, Chondrocytes, Chondrocytes - cytology, Chondrocytes - metabolism, Chondrocytes - pathology, chondrogenic potential, Collagen (type I), Collagen Type I - genetics, Collagen Type I - metabolism, Collagen Type II - genetics, Collagen Type II - metabolism, Collagenase 3, Culture, Degeneration, Experiments, Extracellular matrix, Gene Expression, Gluconacetobacter - metabolism, human cartilage, Humans, Hydrogels, Hydrogels - chemistry, Matrix Metalloproteinase 13 - genetics, Matrix Metalloproteinase 13 - metabolism, Morphology, Osteoarthritis, Pellets, Polystyrene resins, Polystyrenes - chemistry, SOX9 Transcription Factor - genetics, SOX9 Transcription Factor - metabolism, Tissue engineering, Transplants & implants