Details

Autor(en) / Beteiligte

• Kasai, Takatoshi
• Suga, Hidetaka
• Sakakibara, Mayu
• Ozone, Chikafumi
• Matsumoto, Ryusaku
• Kano, Mayuko
• Mitsumoto, Kazuki
• Ogawa, Koichiro
• Kodani, Yu
• Nagasaki, Hiroshi
• Inoshita, Naoko
• Sugiyama, Mariko
• Onoue, Takeshi
• Tsunekawa, Taku
• Ito, Yoshihiro
• Takagi, Hiroshi
• Hagiwara, Daisuke
• Iwama, Shintaro
• Goto, Motomitsu
• Banno, Ryoichi
• Takahashi, Jun
• Arima, Hiroshi

Titel

Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells

Ist Teil von

• Cell reports (Cambridge), 2020-01, Vol.30 (1), p.18-24.e5

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• The pituitary is a major hormone center that secretes systemic hormones responding to hypothalamus-derived-releasing hormones. Previously, we reported the independent pituitary induction and hypothalamic differentiation of human embryonic stem cells (ESCs). Here, a functional hypothalamic-pituitary unit is generated using human induced pluripotent stem (iPS) cells in vitro. The adrenocorticotropic hormone (ACTH) secretion capacity of the induced pituitary reached a comparable level to that of adult mouse pituitary because of the simultaneous maturation with hypothalamic neurons within the same aggregates. Corticotropin-releasing hormone (CRH) from the hypothalamic area regulates ACTH cells similarly to our hypothalamic-pituitary axis. Our induced hypothalamic-pituitary units respond to environmental hypoglycemic condition in vitro, which mimics a life-threatening situation in vivo, through the CRH-ACTH pathway, and succeed in increasing ACTH secretion. Thus, we generated powerful hybrid organoids by recapitulating hypothalamic-pituitary development, showing autonomous maturation on the basis of interactions between developing tissues. [Display omitted] •Hypothalamic-pituitary functional units can be generated from 3D-cultured human iPS cells•Maturation of induced pituitary through simultaneous culture with hypothalamic neurons•ACTH secretion ability of induced pituitary is comparable with adult mice•Induced pituitary cells respond to low-glucose stimulation through the CRH-ACTH pathway Kasai et al. generate a functional hypothalamic-pituitary unit using human iPS cells. The ACTH secretion capacity of induced pituitary cells reaches the same level as adult mice anterior pituitary cells. The hypothalamic-pituitary unit responds to environmental stimulation such as low glucose through the CRH-ACTH pathway.