Details

Autor(en) / Beteiligte

• Miller, Walter J
• Kayton, Mark L
• Patton, Angela
• O'Connor, Sarah
• He, Mei
• Vu, Huan
• Baibakov, Galina
• Lorang, Dominique
• Knezevic, Vladimir
• Kohn, Elise
• Alexander, H Richard
• Stirling, David
• Payvandi, Faribourz
• Muller, George W
• Libutti, Steven K

Titel

A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

Ist Teil von

• Journal of translational medicine, 2004-01, Vol.2 (1), p.4-4

Ort / Verlag

England: BioMed Central Ltd

Erscheinungsjahr

2004

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Reliable quantitative evaluation of molecular pathways is critical for both drug discovery and treatment monitoring. We have modified the CAM assay to quantitatively measure vascular density, endothelial proliferation, and changes in protein expression in response to anti-angiogenic and pro-angiogenic agents. This improved CAM assay can correlate changes in vascular density with changes seen on a molecular level. We expect that these described modifications will result in a single in vivo assay system, which will improve the ability to investigate molecular mechanisms underlying the angiogenic response.